Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism
Open Access
- 2 August 2019
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 9 (8), 335
- https://doi.org/10.3390/biom9080335
Abstract
Insulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. Annulohypoxylon annulatum is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of A. annulatum. The aim of this study is to evaluate the effects of hypoxylonol F isolated from A. annulatum on insulin secretion in INS-1 pancreatic β-cells and demonstrate the molecular mechanisms involved. Glucose-stimulated insulin secretion (GSIS) values were evaluated using a rat insulin ELISA kit. Moreover, the expression of proteins related to pancreatic β-cell metabolism and insulin secretion was evaluated using Western blotting. Hypoxylonol F isolated from A. annulatum was found to significantly enhance glucose-stimulated insulin secretion without inducing cytotoxicity. Additionally, hypoxylonol F enhanced insulin receptor substrate-2 (IRS-2) levels and activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. Interestingly, it also modulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and pancreatic and duodenal homeobox 1 (PDX-1). Our findings showed that A. annulatum and its bioactive compounds are capable of improving insulin secretion by pancreatic β-cells. This suggests that A. annulatum can be used as a therapeutic agent to treat diabetes.Funding Information
- Korea Institute of Science and Technology (2Z05610)
- National Research Foundation of Korea (2017R1C1B5015841, 2019R1F1A1059173)
This publication has 37 references indexed in Scilit:
- Geniposide Regulates Glucose-Stimulated Insulin Secretion Possibly through Controlling Glucose Metabolism in INS-1 CellsPLOS ONE, 2013
- Peroxisome Proliferator-activated Receptor γ (PPARγ) and Its Target Genes Are Downstream Effectors of FoxO1 Protein in Islet β-Cells: MECHANISM OF β-CELL COMPENSATION AND FAILUREOnline Journal of Public Health Informatics, 2013
- Cocoa flavonoids improve insulin signalling and modulate glucose production via AKT and AMPK in HepG2 cellsMolecular Nutrition & Food Research, 2013
- PPAR-γ Activation Increases Insulin Secretion through the Up-regulation of the Free Fatty Acid Receptor GPR40 in Pancreatic β-CellsPLOS ONE, 2013
- A review of the efficacy and safety of oral antidiabetic drugsExpert Opinion on Drug Safety, 2012
- Effects of pioglitazone and rosiglitazone combined with metformin on body weight in people with diabetesDiabetes, Obesity and Metabolism, 2009
- Pdx1 and other factors that regulate pancreatic β‐cell survivalDiabetes, Obesity and Metabolism, 2009
- Physiogenomic comparison of edema and BMI in patients receiving rosiglitazone or pioglitazoneClinica Chimica Acta; International Journal of Clinical Chemistry, 2009
- Dehydroepiandrosterone increases β‐cell mass and improves the glucose‐induced insulin secretion by pancreatic islets from aged ratsFEBS Letters, 2005
- Type 2 Diabetes-a Matter of ß-Cell Life and Death?Science, 2005