CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae
Open Access
- 21 August 2007
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 65 (6), 1493-1502
- https://doi.org/10.1111/j.1365-2958.2007.05883.x
Abstract
After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation–reacylation cycle catalysed by Per1p and Gup1p. Furthermore the diacylglycerol moiety of the yeast GPI anchor can also be replaced by ceramides. CWH43 of yeast is homologous to PGAP2, a gene that recently was implicated in a similar deacylation reacylation cycle of GPI proteins in mammalian cells, where PGAP2 is required for the reacylation of monoradylglycerol-type GPI anchors. Here we show that mutants lacking CWH43 are unable to synthesize ceramide-containing GPI anchors, while the replacement of C18 by C26 fatty acids on the primary diacylglycerol anchor by Per1p and Gup1p is still intact. CWH43 contains the COG3568 metal hydrolase motif, which is found in many eukaryotic and prokaryotic enzymes. The conserved His 802 residue of this motif was identified as being essential for ceramide remodelling. Ceramide remodelling is not required for the normal integration of GPI proteins into the cell wall. All remodelling reactions are dependent on prior removal of the inositol-linked fatty acid by Bst1p.Keywords
This publication has 35 references indexed in Scilit:
- Multiple functions of inositolphosphorylceramides in the formation and intracellular transport of glycosylphosphatidylinositol-anchored proteins in yeastBiochemical Society Symposium, 2007
- Fatty Acid Remodeling of GPI-anchored Proteins Is Required for Their Raft AssociationMolecular Biology of the Cell, 2007
- A global topology map of the Saccharomyces cerevisiae membrane proteomeProceedings of the National Academy of Sciences of the United States of America, 2006
- GUP1ofSaccharomyces cerevisiaeEncodes anO-Acyltransferase Involved in Remodeling of the GPI AnchorMolecular Biology of the Cell, 2006
- Exploration of the Function and Organization of the Yeast Early Secretory Pathway through an Epistatic Miniarray ProfileCell, 2005
- Drag&Drop cloning in yeastGene, 2005
- A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettesYeast, 2004
- Global analysis of protein localization in budding yeastNature, 2003
- Getting started with yeastMethods in Enzymology, 2002
- Primary Structure of CD52Online Journal of Public Health Informatics, 1995