Molecular Epidemiological Investigation of Hepatitis E Virus in Rattus norvegicus in Dali, Yunnan
- 1 January 2020
- journal article
- Published by Hans Publishers in Asian Case Reports in Veterinary Medicine
- Vol. 09 (02), 19-24
- https://doi.org/10.12677/acrpvm.2020.92003
Abstract
目的:了解云南省大理州捕获的褐家鼠中戊型肝炎病毒(Hepatitis E Virus, HEV)感染流行率。方法:采用逆转录巢式聚合酶链反应(RT-nested PCR)技术及组织病理学观察(H.E.),对大理州55份褐家鼠组织样品进行检测。结果:本次实验检测55份褐家鼠组织样品,其中肝脏中HEV阳性率为18.1% (10/55),肾脏中HEV阳性率为10.9% (6/55),且均无性别差异。组织病理学观察发现HEV感染会引起鼠组织损伤。结论:云南省大理州褐家鼠中存在戊型肝炎病毒感染,这将有利于中国戊型肝炎病毒的了解与防治。 Objective: To investigate the prevalence of Hepatitis E Virus (HEV) infection in Rattus norvegicus captured from Dali prefecture, Yunnan province. Methods: 55 samples of Rattus norvegicus from Dali city were tested by Reverse Transcription nested Polymerase Chain Reaction (RT-nPCR) and Histopathological observation (H.E.). Results: Fifty-five Rattus norvegicus tissue samples were tested in this study, among which the HEV positive rate in the liver was 18.1% (10/55) and the HEV positive rate in the kidney was 10.9% (6/55). Histopathological observations showed that HEV infection caused tissue damage in mice. Conclusion: There is hepatitis E virus infection in Rattus norvegicus in Dali prefecture of Yunnan province, which is beneficial to the understanding and prevention of hepatitis E virus in China.This publication has 27 references indexed in Scilit:
- In vivo evidence for ribavirin-induced mutagenesis of the hepatitis E virus genomeGut, 2016
- Proposed reference sequences for hepatitis E virus subtypesJournal of General Virology, 2016
- Chronic Infection With Camelid Hepatitis E Virus in a Liver Transplant Recipient Who Regularly Consumes Camel Meat and MilkGastroenterology, 2016
- Hepatitis E virus infection: Epidemiology and treatment implicationsWorld Journal of Virology, 2015
- Bats Worldwide Carry Hepatitis E Virus-Related Viruses That Form a Putative Novel Genus within the Family HepeviridaeJournal of Virology, 2012
- Hepatitis EThe Lancet, 2012
- The global burden of hepatitis E virus genotypes 1 and 2 in 2005Journal of Hepatology, 2012
- Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotypeJournal of General Virology, 2011
- Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosisReviews in Medical Virology, 2005
- Thermal Stability of Hepatitis E VirusThe Journal of Infectious Diseases, 2005