Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
Open Access
- 12 August 2022
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 23 (16), 9023
- https://doi.org/10.3390/ijms23169023
Abstract
The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate copper metabolism in a way that affects redox homeostasis, thus contributing to progression of metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and decreased metallothionein level, while rendering the level of copper importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased renal copper level, and modulated renal copper metabolism as to preserve vital cellular function including mitochondrial energy production and antioxidative defense, at the expense of intracellular copper storage.Funding Information
- Ministry of Education, Science, and Technological Development of the Republic of Serbia (451-03-68/2022-14/ 200007 and 451-03-68/2022-14/200053)
This publication has 83 references indexed in Scilit:
- High fructose feeding induces copper deficiency in Sprague–Dawley rats: A novel mechanism for obesity related fatty liverJournal of Hepatology, 2012
- Decreased Erythrocyte CCS Content is a Biomarker of Copper Overload in RatsInternational Journal of Molecular Sciences, 2010
- Metabolic Effects of Fructose and the Worldwide Increase in ObesityPhysiological Reviews, 2010
- Copper-dependent Recycling of hCTR1, the Human High Affinity Copper TransporterOnline Journal of Public Health Informatics, 2009
- The copper transporter Ctr1 contributes to cisplatin uptake by renal tubular cells during cisplatin nephrotoxicityAmerican Journal of Physiology-Renal Physiology, 2009
- Hypothesis: Could Excessive Fructose Intake and Uric Acid Cause Type 2 Diabetes?Endocrine Reviews, 2009
- Biomarkers of copper status: a brief updateBritish Journal of Nutrition, 2008
- How reliable and robust are current biomarkers for copper status?British Journal of Nutrition, 2007
- Human kidney diamine oxidase: heterologous expression, purification, and characterizationJBIC Journal of Biological Inorganic Chemistry, 2002
- Glutathione reductase test with whole blood, a convenient procedure for the assessment of the riboflavin status in humansCellular and Molecular Life Sciences, 1974