Comparison of the Mechanical Properties Between the Convex and Concave Inner/Apical Surfaces of the Developing Cerebrum
Open Access
- 23 July 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
The inner/apical surface of the embryonic brain wall is important as a major site for cell production by neural progenitor cells (NPCs). We compared the mechanical properties of the apical surfaces of two neighboring but morphologically distinct cerebral wall regions in mice from embryonic day (E) 12 to E14. Through indentation measurement using atomic force microscopy (AFM), we first found that Young’s modulus was higher at a concave-shaped apical surface of the pallium than at a convex-shaped apical surface of the ganglionic eminence (GE). Further AFM analysis suggested that contribution of actomyosin as revealed with apical-surface softening by blebbistatin and stiffness of dissociated NPCs were both comparable between pallium and GE, not accounting for the differential apical surface stiffness. We then found that the density of apices of NPCs was greater, with denser F-actin meshwork, in the apically stiffer pallium than in GE. A similar correlation was found between the decreasing NPC apices density between E12 and E14 and the declining apical-surface stiffness in the same period in both the pallium and the GE. Thus, one plausible explanation for the observed difference (pallium>GE) in apical surface stiffness may be differential densification of NPC apices. In laser ablation onto the apical surface, the convex-shaped GE apical surface showed quicker recoils of edges than the pallial apical surface did, with a milder inhibition of recoiling by blebbistatin than in pallium. This greater pre-stress in GE may provide an indication for how the initially apically concave wall then becomes an apically convex “eminence”.Funding Information
- Japan Society for the Promotion of Science (16H02457, 21H00363, 21H02656)
This publication has 26 references indexed in Scilit:
- Villification: How the Gut Gets Its VilliScience, 2013
- TAG-1–assisted progenitor elongation streamlines nuclear migration to optimize subapical crowdingNature Neuroscience, 2013
- Amplification of progenitors in the mammalian telencephalon includes a new radial glial cell typeNature Communications, 2013
- Atomic force microscopy and its contribution to understanding the development of the nervous systemCurrent Opinion in Genetics & Development, 2011
- Neural Progenitor Nuclei IN MotionNeuron, 2010
- Myosin II is required for interkinetic nuclear migration of neural progenitorsProceedings of the National Academy of Sciences of the United States of America, 2009
- Development of three‐dimensional architecture of the neuroepithelium: Role of pseudostratification and cellular ‘community’Development, Growth & Differentiation, 2008
- Matrix Elasticity Directs Stem Cell Lineage SpecificationCell, 2006
- Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell responseBiomaterials, 2005
- Calibration of atomic-force microscope tipsReview of Scientific Instruments, 1993