Effects of Two Starch Synthase IIa Isoforms on Grain Components and Other Grain Traits in Barley
- 22 January 2021
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 69 (4), 1206-1213
- https://doi.org/10.1021/acs.jafc.0c05445
Abstract
Starch biosynthesis in cereal crops is a complex pathway regulated by multiple starch synthetic enzymes. Starch synthase IIa (SSIIa) is well-known to be one of the major starch synthases and is very important in amylopectin biosynthesis. It has significant effects on grain composition and kernel traits. However, there are few reports on the association of natural variation of SSIIa in barley and grain composition and characteristics. In this work, two SSIIa isoforms were first identified as SSIIaH and SSIIaL by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, mass spectrometry, and Western blotting. Sequence analysis of the SSIIa gene demonstrated that a 33 bp insertion coding a peptide of APPSSVVPAKK caused different SSIIa, e.g., SSIIaH and SSIIaL. Based on this molecular difference, a polymerase chain reaction marker was developed, which could be used to screen different SSIIa genotypes easily. Kernel hardness of SSIIaL genotypes was significantly higher than that of SSIIaH Chinese barley cultivars. The proportion of SSIIaL genotypes was extremely low in Australian barley cultivars (5/24) and much higher in Tibetan hull-less barley cultivars (46/74), consistent with the end-use requirements of barley grain. This study provided new information in barley endosperm starch synthesis and indicated that it is valuable for choosing the preferred SSIIa genotype according to the end-use requirements.Funding Information
- State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement
- Tibet Academy of Agriculture and Animal Sciences (XZNKY-2020-C-007)
- Second Tibetan Plateau Scientific Expedition and Research Program (2019QZKK0303)
This publication has 41 references indexed in Scilit:
- SNP in starch biosynthesis genes associated with nutritional and functional properties of riceScientific Reports, 2012
- Starch granule‐associated proteins of hull‐less barley (Hordeum vulgare L.) from the Qinghai‐Tibet Plateau in ChinaJournal of the Science of Food and Agriculture, 2011
- Identification of quantitative trait loci for β-glucan concentration in barley grainJournal of Cereal Science, 2008
- Barley for food: Characteristics, improvement, and renewed interestJournal of Cereal Science, 2008
- Effects of Variations in Starch Synthase on Starch Properties and Eating Quality of RicePlant Production Science, 2008
- A Review of the Effects of Barley beta-Glucan on Cardiovascular and Diabetic RiskCereal Foods World, 2006
- Single-nucleotide polymorphisms in rice starch synthase IIa that alter starch gelatinisation and starch association of the enzymeFunctional Plant Biology, 2005
- Natural variation in rice starch synthase IIa affects enzyme and starch propertiesFunctional Plant Biology, 2004
- Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel propertiesThe Plant Journal, 2003
- Evaluation of the 40 mg Swelling Test for Measuring Starch FunctionalityStarch ‐ Stärke, 2001