Exogenous Oleic Acid and Palmitic Acid Improve Boar Sperm Motility via Enhancing Mitochondrial Β-Oxidation for ATP Generation
Open Access
- 31 March 2020
- Vol. 10 (4), 591
- https://doi.org/10.3390/ani10040591
Abstract
It takes several hours for mammalian sperm to migrate from the ejaculation or insemination site to the fertilization site in the female reproductive tract in which glucose, amino acids, and fatty acids are regarded as the primary substrates for ATP generation. The present study was designed to investigate whether oleic acid and palmitic acid were beneficial to boar sperm in vitro; and if yes, to elucidate the mechanism that regulates sperm motility. Therefore, the levels of oleic acid and palmitic acid, motility, membrane integrity, acrosome integrity, and apoptosis of sperm were evaluated. Moreover, the enzymes involved in mitochondrial β-oxidation (CPT1: carnitine palmitoyltransferase 1; ACADVL: long-chain acyl-coenzyme A dehydrogenase) were detected with immunofluorescence and Western blotting. Consequently, the ATP content and the activities of CPT1, ACADVL, malate dehydrogenase (MDH), and succinate dehydrogenase (SDH) were also measured. We observed that CPT1 and ACADVL were expressed in boar sperm and localized in the midpiece. The levels of oleic acid and palmitic acid were decreased during storage at 17 °C. The addition of oleic acid and palmitic acid significantly increased sperm motility, progressive motility, straight-line velocity (VSL), membrane integrity, and acrosome integrity with a simultaneous decrease in sperm apoptosis after seven days during storage. When sperm were incubated with oleic acid and palmitic acid at 37 °C for 3 h, the activities of CPT1 and ACADVL, the ATP level, the mitochondrial membrane potential, the activities of MDH and SDH, as well as sperm motility patterns were significantly increased compared to the control (p < 0.05). Moreover, the addition of etomoxir to the diluted medium in the presence of either oleic acid or palmitic acid and the positive effects of oleic acid and palmitic acid were counteracted. Together, these data suggest that boar sperm might utilize oleic acid and palmitic acid as energy substrates for ATP production via β-oxidation. The addition of these acids could improve sperm quality.Keywords
This publication has 39 references indexed in Scilit:
- Human Sperm Tail Proteome Suggests New Endogenous Metabolic PathwaysMolecular & Cellular Proteomics, 2013
- Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation successAndrology, 2012
- Energy metabolism of cancer: Glycolysis versus oxidative phosphorylation (Review)Oncology Letters, 2012
- Targeting fatty acid and carbohydrate oxidation — A novel therapeutic intervention in the ischemic and failing heartBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2011
- The enzymology of mitochondrial fatty acid beta‐oxidation and its application to follow‐up analysis of positive neonatal screening resultsJournal of Inherited Metabolic Disease, 2010
- Nutrient-sensitized screening for drugs that shift energy metabolism from mitochondrial respiration to glycolysisNature Biotechnology, 2010
- New insights concerning the role of carnitine in the regulation of fuel metabolism in skeletal muscleJournal Of Physiology-London, 2007
- Identification of gene products present in Triton X‐100 soluble and insoluble fractions of human spermatozoa lysates using LC‐MS/MS analysisProteomics – Clinical Applications, 2007
- Sperm transport in the female reproductive tractHuman Reproduction Update, 2005
- FLAGELLAR MOVEMENT AND ADENOSINE TRIPHOSPHATASE ACTIVITY IN SEA URCHIN SPERM EXTRACTED WITH TRITON X-100The Journal of cell biology, 1972