Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
Open Access
- 1 March 2019
- journal article
- research article
- Published by Taylor & Francis Ltd in International Journal of Nanomedicine
- Vol. ume 14, 1969-1978
- https://doi.org/10.2147/ijn.s188235
Abstract
Background: Tremendous growth in nanotechnology has opened up new frontiers in fundamental and applied aspects, including the synthesis of nanoscale matter and understanding/utilizing its exotic physicochemical and optoelectronic properties. Green-synthesis methods employing either biological microorganisms or plant extracts have emerged as a simple and alternative to chemical synthesis. Methods: In our present study, we aimed to synthesize silver nanoparticles (AgNPs) in combination with an aqueous extract of Coptis chinensis (CC) using a suitable ecofriendly green-synthesis way. Results: In our results, ultraviolet-visible spectroscopy revealed a near-absorbance peak at 450 nm, which confirmed the AgNP synthesis. The crystalline nature of the AgNPs was revealed with X-ray diffraction. Transmission electron-microscopy analysis showed spherically dispersed nanoparticles of 6–45 nm diameter. We analyzed the elementary mechanism across A549 lung carcinoma cells ahead of treatment with doses of CC-AgNPs (10 µg/mL and 25 µg/mL). The antiproliferative effect of CC-AgNPs revealed a significant decline in cell viability. Antibacterial assays with both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria exhibited a higher zone of inhibition against S. aureus. Conclusion: Furthermore, CC-AgNPs regulated apoptosis using the intrinsic pathway to inhibit A549-cell proliferation. Proliferation migration and invasion were notably inhibited by CC-AgNPs, which promoted apoptosis in lung adenocarcinoma cells by regulating the apoptotic pathway.Keywords
This publication has 35 references indexed in Scilit:
- Targeting apoptosis pathways by natural compounds in cancer: Marine compounds as lead structures and chemical tools for cancer therapyCancer Letters, 2013
- Cancer statistics, 2013CA: A Cancer Journal for Clinicians, 2013
- Multipolar functions of BCL-2 proteins link energetics to apoptosisTrends in Cell Biology, 2012
- Inhibitory activities of the alkaloids from Coptidis Rhizoma against aldose reductaseArchives of Pharmacal Research, 2008
- Evaluation of the anti-proliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cellsJournal of Ethnopharmacology, 2006
- Hypoglycemic and Hypocholesterolemic Effects of Coptis chinensis Franch InflorescencePlant Foods for Human Nutrition, 2006
- Extraction of berberine from rhizome of Coptis chinensis Franch using supercritical fluid extractionJournal of Pharmaceutical and Biomedical Analysis, 2006
- Artepillin C in Brazilian propolis induces G0/G1 arrest via stimulation of Cip1/p21 expression in human colon cancer cellsMolecular Carcinogenesis, 2005
- Protective role of Coptidis Rhizoma alkaloids against peroxynitrite-induced damage to renal tubular epithelial cellsJournal of Pharmacy and Pharmacology, 2005
- Antioxidant activity of anti-inflammatory plant extractsLife Sciences, 2002