Abstract
Dactylogyrus petruschewskyi Gussev, 1955 (Monogenea: Dactylogyridae) was found infecting the gills of Megalobrama amblycephala Yih, 1955 (Cypriniformes: Cyprinidae: Cultrinae) that was sampled from the Ono River flowing into Lake Kasumigaura in Ibaraki Prefecture, central Japan. This is the first record of this monogenean from Japan, and this parasite was most likely introduced and established along with M. amblycephala from China into Japan. The 28S rDNA sequence of D. petruschewskyi is available in the database but not based on a published study. We analyzed molecular data based on newly collected specimens, and the obtained sequence corresponded to the available sequence.

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