Association of C677T MTHFR and G20210A FII prothrombin polymorphisms with susceptibility to myocardial infarction
- 13 July 2016
- journal article
- Published by Spandidos Publications in Biomedical Reports
- Vol. 5 (3), 361-366
- https://doi.org/10.3892/br.2016.717
Abstract
Myocardial infarction (MI) is a common complex pathology, localized in the main leading causes of mortality worldwide. It is the result of the interaction of genetic and environmental factors. The aim of the present study was to investigate the potential association of C677T 5,10-methylenetetrahydrofolate reductase (MTHFR) (rs1801133) and G20210A factor II prothrombin (FII) (rs1799963) polymorphisms with the susceptibility of MI. Following extraction by the standard salting-out procedure, DNA samples of 100 MI patients and 182 apparently healthy controls were genotyped by polymerase chain reaction‑restriction fragment length polymorphism using HinfI and HindIII restriction enzymes, respectively. The results show a significant association of the G20210T FII polymorphism with the MI risk. The frequencies of the heterozygote genotype GA, homozygous mutated AA and the G20210A allele was higher among patients compared to controls (GA: 59 vs. 5.5%, P<0.001; AA: 10 vs. 0%, P=0.003; and 20210A: 39.5 vs. 2.7%, P<0.003), suggesting that this polymorphism may be a potential genetic marker for MI. No significant association was observed between the C677T MTHFR and MI occurrence, and there was more heterozygote CT in the patient group compared to the controls. As a multifactorial disease, the development of MI may be the result of numerous factors that influence synergistically its occurrence. Thus, further studies are merited to try to better assess these associations (gene-gene and gene-environment interactions).Keywords
This publication has 43 references indexed in Scilit:
- Original research Genetic variability and the risk of myocardial infarction in Poles under 45 years of ageVideosurgery and Other Miniinvasive Techniques, 2010
- The genetics of venous thromboembolismThrombosis and Haemostasis, 2009
- Association of Methylenetetrahydrofolate Reductase (MTHFR-677 and MTHFR-1298) Genetic Polymorphisms with Occlusive Artery Disease and Deep Venous Thrombosis in MacedoniansCroatian Medical Journal, 2008
- Factor VLeiden and Prothrombin G20210A Gene Polymorphisms in Patients with Coronary Artery DiseaseYonsei Medical Journal, 2008
- No interaction between factor V Leiden and hyperhomocysteinemia or MTHFR 677TT genotype in venous thrombosisThrombosis and Haemostasis, 2007
- Acute stroke in relation to homocysteine and methylenetetrahydrofolate reductase gene polymorphismsActa Neurologica Scandinavica, 2006
- Homocysteine metabolism in families from southern Italy with neural tube defects: role of genetic and nutritional determinantsPrenatal Diagnosis, 2005
- Meta-analysis of MTHFR 677C→ T polymorphism and coronary heart disease: does totality of evidence support causal role for homocysteine and preventive potential of folate?BMJ, 2005
- Hyperhomocysteinemia Is Inversely Related With Left Ventricular Ejection Fraction and Predicts Cardiovascular Mortality in High-Risk Coronary Artery Disease HypertensivesArteriosclerosis, Thrombosis, and Vascular Biology, 2005
- A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductaseNature Genetics, 1995