Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis
Open Access
- 3 March 2023
- journal article
- research article
- Published by Medknow in Asian Journal of Andrology
- Vol. 25 (5), 591-597
- https://doi.org/10.4103/aja2022115
Abstract
We examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF; n = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), transmission electronic microscopy (TEM), biochemical analysis, and sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL) assay. Azoospermia was diagnosed in 83 (89.2%) patients. The other 10 (10.8%) patients were found to be nonazoospermic and showed various spermatological diagnoses (asthenozoospermia, n = 2; asthenoteratozoospermia, n = 3; oligoasthenozoospermia, n = 1; oligoasthenoteratozoospermia, n = 3; and normozoospermia, n = 1) with no specific morphological abnormalities. Oligospermia was detected in 89.2% azoospermic and 30.0% nonazoospermic patients. Low seminal pH (30%) of spermatozoa with noncondensed (“immature”) chromatin was revealed in 2 of 6 nonazoospermic semen samples analyzed by TEM.Keywords
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