The Effect of Hormonal Treatment on Cell Viability in F98 Cell Line
- 12 March 2020
- journal article
- Published by Van Veterinary Journal in Van Veterinary Journal
- Vol. 31 (1), 46-49
- https://doi.org/10.36483/vanvetj.620421
Abstract
The aim of the present study is to investigate the effects of three different steroid hormones; 17 β estradiol, Diethylstilbestrol and progesterone on cell viability in F98 glioblastoma cells. F98 glioblastoma cells were treated with different concentrations of Progesterone (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) and 17β estradiol (0.01, 0.1, 1, 10 μM)) for 24, 48 and 72 hours and MTT assay was applied to determine thecell viability. Progesterone inhibits glioblastoma cell growth in a dose and time dependent manner. Antiproliferative effect of 17 β estradiol was observed at low doses. Biphasic distribution was observed in decreasing cell viability in DES applications. These results suggest that Progesterone, 17β estradiol and DES can inhibit the proliferation of glioblastoma cells. However, further study is necessary to identify the pathways involved.Bu çalışmada 17 β östradiol, Dietilsitilbesterol ve progesterone gibi steroid hormonların F98 glioblostomo hücre hatlarında hücre canlılığı üzerine etkilerinin araştırılması amaçlanmıştır. F98 glioblastoma hücrelerine farlı dozlarda Progesteron (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) ve 17β estradiol (0.01, 0.1, 1, 10 μM) 24, 48 ve 72 saat süre ile uygulanmış ve hücre canlılığının belirlenmesi amacıyla MTT hücre canlılık testi uygulanmıştır. Progesteron glioblostoma hücrelerinin büyümesini doz ve zaman bağımlı olarak inhibe etmiştir. 17 β estradiol düşük dozlarda antiproliferatif etki göstermiştir. DES uygulamaları hücre canlılığı üzerinde iki yönlü etki göstermiştir. Elde edilen sonular Progesterone, 17β estradiol ve DES’in glioblostoma hücrelerinde hücre çoğalmasını inhibe ettiğini göstermektedir. Ancak bununla ilgili yolakların belirlenmesi için daha fazla çalışma yapılmasına ihtiyaç vardır.Keywords
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