Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.
Open Access
- 27 November 2015
- journal article
- research article
- Published by International Union of Crystallography (IUCr) in Acta crystallographica. Section D, Structural biology
- Vol. 71 (12), 2519-25
- https://doi.org/10.1107/S139900471501857X
Abstract
Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.Keywords
This publication has 43 references indexed in Scilit:
- Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction dataActa crystallographica. Section D, Structural biology, 2013
- Lipidic phase membrane protein serial femtosecond crystallographyNature Methods, 2012
- ANODE: anomalous and heavy-atom density calculationJournal of Applied Crystallography, 2011
- Overview of theCCP4 suite and current developmentsActa crystallographica. Section D, Structural biology, 2011
- Femtosecond X-ray protein nanocrystallographyNature, 2011
- Experimental phasing with SHELXC/D/E: combining chain tracing with density modificationActa crystallographica. Section D, Structural biology, 2010
- PHENIX: a comprehensive Python-based system for macromolecular structure solutionActa crystallographica. Section D, Structural biology, 2010
- XDSActa crystallographica. Section D, Structural biology, 2010
- TheBuccaneersoftware for automated model building. 1. Tracing protein chainsActa crystallographica. Section D, Structural biology, 2006
- Coot: model-building tools for molecular graphicsActa crystallographica. Section D, Structural biology, 2004