Еvaluation of the genetic diversity of varieties of camelina (Сamelina sativa l.) using SSR markers

Abstract

Relevance. Camelina (Camelina sativa L.) is a geographically widespread oilseed crop characterized by a high content of unsaturated fatty acids in seed oil and resistance to most stress abiotic and biotic factors. The prospect for the development of camelina culture in agriculture is associated with the production of biofuels and a wide range of technical oils. The creation of new specialized highly productive varieties of camelina is associated with the tasks of the chemical, medical and food industries. Increasing the efficiency of the breeding process needs development of methods for the assessing and selecting of genetic material (lines, cultivars etc.). The study of the genetic diversity of a culture using DNA marking, including the microsatellite SSR markers, is considered as an effective way of the pre-breeding stage of breeding work. The aim of this study was to study the polymorphism and phylogenetic relationships of camelina cultivars using SSR markers.Methods. The object of the study was 18 varieties of camelina of various breeding origin, included in the State Register of Varieties of the Russian Federation. Genetic analysis was carried out by PCR using a set of 8 SSR-markers, followed by detection of products on a genetic analyzer.Results. During the study, 40 alleles were identified, with rather high indicators of the level of polymorphism. A specific marker associated with the winter form of life of camelina was revealed. Cluster analysis with the construction of a dendrogram of genetic similarity showed significant differences in the studied samples. The varieties were divided into two separate clusters — winter and spring forms of camelina. In each of the clusters, varieties were grouped mainly by origin (originator). Further development and use of DNA-marking methods will contribute to increasing the efficiency of the breeding process and the formation of a system of genetic certification of oilseeds.