Shared B cell memory to coronaviruses and other pathogens varies in human age groups and tissues
Open Access
- 14 May 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 372 (6543), 738-741
- https://doi.org/10.1126/science.abf6648
Abstract
Vaccination and infection promote the formation, tissue distribution, and clonal evolution of B cells, which encode humoral immune memory. We evaluated convergent antigen-specific antibody genes of similar sequences shared between individuals in pediatric and adult blood, and deceased organ donor tissues. B cell memory varied for different pathogens. Polysaccharide antigen-specific clones were not exclusive to the spleen. Adults had higher clone frequencies and greater class-switching in lymphoid tissues than blood, while pediatric blood had abundant class-switched convergent clones. Consistent with reported serology, pre-pandemic children had class-switched convergent clones to SARS-CoV-2 with weak cross-reactivity to other coronaviruses, while adult blood or tissues showed few such clones. The results highlight the prominence of early childhood B cell clonal expansions and cross-reactivity for future responses to novel pathogens.Funding Information
- National Cancer Institute (U54CA260517)
- National Institute of Allergy and Infectious Diseases (R01AI127877)
- National Institute of Allergy and Infectious Diseases (R01AI130398)
- National Institute of Allergy and Infectious Diseases (U19AI057229)
- National Institute of Allergy and Infectious Diseases (U19AI090019)
- Crown Family Foundation
- this (NIH R01 HD063142)
This publication has 87 references indexed in Scilit:
- IgBLAST: an immunoglobulin variable domain sequence analysis toolNucleic Acids Research, 2013
- CD-HIT: accelerated for clustering the next-generation sequencing dataBioinformatics, 2012
- Trends in Meningococcal Disease in the United States Military, 1971–2010Emerging Infectious Diseases, 2012
- Pandemic H1N1 influenza vaccine induces a recall response in humans that favors broadly cross-reactive memory B cellsProceedings of the National Academy of Sciences of the United States of America, 2012
- H3N2 Influenza Infection Elicits More Cross-Reactive and Less Clonally Expanded Anti-Hemagglutinin Antibodies Than Influenza VaccinationPLOS ONE, 2011
- Epitope-Specific Human Influenza Antibody Repertoires Diversify by B Cell Intraclonal Sequence Divergence and Interclonal ConvergenceThe Journal of Immunology, 2011
- Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infectionThe Journal of Experimental Medicine, 2011
- Secondary Immunization Generates Clonally Related Antigen-Specific Plasma Cells and Memory B CellsThe Journal of Immunology, 2010
- The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutationsMolecular Immunology, 2009
- Human Immunoglobulin Repertoires against Tetanus Toxoid Contain a Large and Diverse Fraction of High-Affinity Promiscuous VH GenesJournal of Molecular Biology, 2009