Comparing The Reference Strains and Turkey Isolates of Bovine Parainfluenza Virus 3 (BPIV3) Detected Around Western Mediterranean Region with Its Amino Acid and Nucleotide Positions

Abstract

One of the major viral pathogens of respiratory system disease complex in cattle, BPIV 3 is a viral agent generally appearing during autumn and winter months in Northern Hemisphere and causing upper respiratory tract infections. Even though the isolates of this virus are in close antigenic relations, it is classified within three different genotypes as BPIV3 Genotype A, BPIV3 Genotype B and BPIV3 Genotype C. In this research, we aimed to compare the strains of BPIV 3 strain circulating around Western Mediterranean region of Turkey and isolated from different regions of this country and the reference strain of BPIV 3, Shipping Fever (SF-4) with amino acid and nucleotide positions. In the study, previously detected BUR/BPIV 3 isolate with M gene region partially analyzed was used. Phylogenetic researches carried out partially on M gene region in Turkey, different BPIV 3 isolates recorded in gene bank and amino acid and nucleotide positions of BPIV 3 strain detected by ourselves were compared. The changes in Turkey strains of BPIV 3 and nucleotide and amino acid positions of the reference strain were revealed. As a result, detecting base and codon differentiations caused by point mutations among BPIV 3 isolates and correspondingly the appearing amino acid changes was considered crucial in terms of revealing the immunization power of the strain to be used in vaccine production and providing the standardization of BPIV 3 molecular detection.