The Biological Faculty of the Saratov State University turns 90 years old in 2021. The first students were enrolled at the Faculty in the 1931–1932 academic year. However, the faculty’s history began long before its opening, when the Department of Botany and the Department of Zoology with Comparative Anatomy were organized in the new Saratov Imperial Nikolaev University in 1909. The article describes important events in the life of the Faculty and the contribution of scientists who stood at the origins of the birth of biological science at Saratov State University and played an important role in the formation and development of the Biological Faculty. A whole galaxy of famous biologists and eminent personalities worked at the university at different times. These are the academicians N. I. Vavilov, N. A. Maksimov, A. A. Richter, corresponding members A. Ya. Gordyagin, S. D. Lvov, professors D. E. Yanishevsky, A. D. Fursaev, I. V. Krasovskaya, V. S. Elpatevskiy, B. K. Fenyuk, P. A. Vunder, A. A. Chiguryaeva, S. S. Khokhlov, M. P. Gnutenko, V. V. Ignatov and others. They founded scientific schools, laid down faculty traditions and set a high standard for biological education, which the modern faculty team strives to maintain to this day.