Telemedicine is an innovation in providing health services to patients, perceptions of technology vary from one individual to another. The level of technology acceptance is something that needs to be researched because it will affect the use of a technology in the company, this is because the received technology will continue to be used in the company and the rejected technology will be a neglected part. There are the important considered criteria for the acceptance of a technology of this research: the adoption of innovation, organizational measurement, business environment, and communication channels. The purpose of this study was to analyze the effect of technology acceptance, organizational size, business environment on the adoption of telemedicine with the influence of the communication channel. The research method is an explanatory research using a quantitative approach. The population in this study was 156 people. The used data analysis method in this research is a Moderated Regression Analysis (MRA). The results from the MRA analysis show that technology acceptance is positively and significantly influenced by communication channels on the adoption of telemedicine. In contrast, the measurement of the organization and the business environment did not have a significant effect on the adoption of telemedicine in the health service industry. The implication of hospital management to increase technology acceptance must pay attention to the advantages of using telemedicine, the suitability of existing technology in hospitals to develop telemedicine, the ease of used technology, the results that seen from the use of telemedicine and it can be tested before being applied. Communication needs to be improved by paying attention to the credibility of the communicator, the content and context of telemedicine, the clarity and continuity of information, the used language is easy to understand and the right communication channel is interpersonal.