Relationship of Breast Cancer Susceptibility with Rs6257 and Rs6258 Polymorphisms in the SHBG Gene
Open Access
- 9 October 2020
- journal article
- research article
- Published by DoNotEdit in Iranian Red Crescent Medical Journal
- Vol. 22 (10)
- https://doi.org/10.32592/ircmj.2020.22.10.105
Abstract
Background: Breast cancer is a hormone-dependent malignancy. Sex hormone-binding globulin (SHBG) is the main transport binding protein for sex steroid hormones in plasma and regulates their accessibility to target cells. Objective: The present study aimed to assess the relationship between rs6257 and rs6258 polymorphisms in the SHBG gene with breast cancer risk. Materials and Methods: This case-control study was conducted on 100 breast cancer patients and 100 healthy control subjects. After extracting DNA from peripheral blood, Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed in SPSS software using the chi-square test and logistic regression to distinguish the difference between the cases and controls in terms of the distribution of allele types and genotypes. Results: The frequencies of Rs6257 were 78%, 17%, 5% in the case group and 88%, 7%, and 5% in control groups for TT, CC, and CT genotypes respectively. The rs6257 CC genotype showed about a 2.721-fold increase in breast cancer (P=0.030, OR:2.721, CI95%:1.075-6.887). This genotype was reversely associated with age, body mass index, menopausal status, and breast cancer. Nonetheless, Rs6258 had no significant correlation with breast cancer risk or other demographic factors. Conclusions: As evidenced by the obtained results, the rs6257 polymorphism may be a useful biomarker associated with breast cancer risk in Iranian women. To confirm the findings of the present study, larger studies are needed in the future.Keywords
This publication has 16 references indexed in Scilit:
- Genetic Variants in Hormone-Related Genes and Risk of Breast CancerPLOS ONE, 2013
- Breast Cancer in Developing Countries: Opportunities for Improved SurvivalJournal of Oncology, 2010
- Genetic susceptibility to breast cancerMolecular Oncology, 2010
- Sex hormone-binding globulin gene expression in the liver: Drugs and the metabolic syndromeMolecular and Cellular Endocrinology, 2010
- Genetic variants of sex hormone-binding globulin and their biological consequencesMolecular and Cellular Endocrinology, 2010
- Genetic polymorphisms are associated with serum levels of sex hormone binding globulin in postmenopausal womenBMC Medical Genetics, 2008
- Identification of Common Variants in the SHBG Gene Affecting Sex Hormone-Binding Globulin Levels and Breast Cancer Risk in Postmenopausal WomenCancer Epidemiology, Biomarkers & Prevention, 2008
- Polymorphisms in estrogen- and androgen-metabolizing genes and the risk of gastric cancerCarcinogenesis: Integrative Cancer Research, 2008
- Sex Hormone-binding Globulin Selectively Modulates Estradiol-regulated Genes in MCF-7 CellsHormone and Metabolic Research, 2007
- Commonly Studied Single-Nucleotide Polymorphisms and Breast Cancer: Results From the Breast Cancer Association ConsortiumJNCI Journal of the National Cancer Institute, 2006