Seven mulberry genotypes, namely: Alf-004, Alf-018, Alf-025, S61-011, S61-019, S54-019 and the Batac were evaluated in terms of biochemical parameters along with leaf yield. Parameters viz; moisture content (MC), total carbohydrate (TC), crude fat (CF), ash, crude protein (CP), Total N,P and K along with leaf yield were analyzed using the ANOVA in Randomized Completely Block Design (RCBD). Further, Evaluation Method Index was used to determine the superior mulberry genotypes. The data revealed that the different genotypes under study vary in their biochemical components where significant variations in all the parameters evaluated except in percent total phosphorus. For the phytochemical analysis, alkaloids, carbohydrates, glycosides, saponins, phytosterols, phenolic compounds, flavonoids and proteins were detected. Based on the evaluation index value, the identified and selected superior genotypes having higher leaf biochemical composition were Alf-004, Alf-018 and Alf-025. Keywords - Biochemistry, open-pollinated mulberry varieties, phytochemical, Randomized Complete Block Design, Philippines, Asia