| Fasciolosis is a common parasitic disease affecting cattle and other ruminants, commonly sheep, and caused by Fasciola hepatica and F. gigantica . The disease is cosmopolitan in distribution and can cause extensive economic losses to the farmers. A cross-sectional study was conducted to determine the prevalence of fasciolosis in commercial cattle farms of Tilottama Municipality, Rupandehi district, Nepal. A total of 270 fresh faecal samples were collected purposively from the study area with different ages, sex, stage, and breeds for examination (sedimentation method) to visualize eggs of Fasciola microscopically. The obtained data were coded and analysed using Microsoft Excel 2016. The overall prevalence of fasciolosis in cattle was found to be 15.56%. Age and sex-wise prevalence was found to be statistically significant (P0.05). Fasciolosis is prevalent moderately among cattle in Tillottoma municipality, which necessitates the study of detailed epidemiology of the disease and effective control strategies to prevent huge economic losses.