Effects of Ranolazine on Carbohydrate Metabolism in the Isolated Perfused Rat Liver
Open Access
- 1 January 2014
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Open Journal of Medicinal Chemistry
- Vol. 04 (04), 87-95
- https://doi.org/10.4236/ojmc.2014.44007
Abstract
The action mechanism of ranolazine, an antiangina drug, could be at least partly metabolic, including inhibition of fatty acid oxidation and stimulation of glucose utilization in the heart. The purpose of the present work was to investigate if ranolazine affects hepatic carbohydrate metabolism. For this purpose, the hemoglobin-free isolated perfused rat liver was used as the experimental system. Ranolazine increased glycolysis and glycogenolysis and decreased gluconeogenesis. These effects were accompanied by an inhibition of oxygen consumption. The drug also changed the redox state of the NAD+-NADH couple. For the cytosol, increased NADH/NAD+ ratios were observed both under glycolytic conditions as well as under gluconeogenic conditions. For the mitochondria, increased NADH/NAD+ ratios were found in the present work in the absence of exogenous fatty acids in contrast with the previous observation of a decreasing effect when the liver was actively oxidizing exogenous oleate. It seems likely that ranolazine inhibits gluconeogenesis and increases glycolysis in consequence of its inhibitory actions on energy metabolism and fatty acid oxidation and by deviating reducing equivalents in favour of its own biotransformation. This is in line with the earlier postulates that ranolazine diminishes fatty acid oxidation, shifting the energy source from fatty acids to glucose.Keywords
This publication has 23 references indexed in Scilit:
- Mechanisms of ranolazine's dual protection against atrial and ventricular fibrillationEP Europace, 2012
- Metabolic fluxes in the liver of rats bearing the Walker‐256 tumour: influence of the circulating levels of substrates and fatty acidsCell Biochemistry and Function, 2007
- Inhibition of free fatty acids metabolism as a therapeutic target in patients with heart failureInternational Journal of Clinical Practice, 2007
- Metabolic effects of carbenoxolone in rat liverJournal of Biochemical and Molecular Toxicology, 2006
- Ranolazine*Cardiology in Review, 2005
- Metabolic Effects of Propofol in the Isolated Perfused Rat LiverBasic & Clinical Pharmacology & Toxicology, 2004
- The urea cycle and related pathways in the liver of Walker-256 tumor-bearing ratsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2004
- Action of quercetin on glycogen catabolism in the rat liverXenobiotica, 2003
- Hepatic metabolism of meal-fed rats: Studies in vivo and in the isolated perfused liverPhysiology & Behavior, 1990
- Stimulation of [1‐14C]oleate oxidation to 14CO2 in isolated rat hepatocytes by vasopressin: effects of Ca2+FEBS Letters, 1980