A network pharmacology strategy combined with in vitro experiments to investigate the potential anti‐inflammatory mechanism of Prunus cerasifera Ehrhart
- 28 September 2022
- journal article
- research article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 46 (12)
- https://doi.org/10.1111/jfbc.14396
Abstract
This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP). LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP. The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP. The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway. ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors kappa B (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1 beta (IL-1 beta) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway. Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases. Practical applications In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects. Thus, the development of anti-inflammatory drugs from natural resources has its application value. In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP). The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-kappa B, MAPK pathway, and NLRP3 inflammasome activation. Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.Keywords
This publication has 35 references indexed in Scilit:
- AKT-targeted anti-inflammatory activity of the methanol extract of Chrysanthemum indicum var. albescensJournal of Ethnopharmacology, 2017
- Ethnopharmacological approach to the herbal medicines of the “Antidotes” in Nikolaos Myrepsos׳ DynameronJournal of Ethnopharmacology, 2015
- Lipopolysaccharide (LPS) promotes osteoclast differentiation and activation by enhancing the MAPK pathway and COX-2 expression in RAW264.7 cellsInternational Journal of Molecular Medicine, 2013
- Antioxidant Activities and Major Anthocyanins of Myrobalan Plum (Prunus cerasifera Ehrh.)Journal of Food Science, 2012
- Inhibition of the NF-κB signaling pathway by the curcumin analog, 3,5-Bis(2-pyridinylmethylidene)-4-piperidone (EF31): Anti-inflammatory and anti-cancer propertiesInternational Immunopharmacology, 2012
- Inflammasomes in health and diseaseNature, 2012
- Nutrient sensing and inflammation in metabolic diseasesNature Reviews Immunology, 2008
- Heme oxygenase 1 and renal ischemia and reperfusion injury: The impact of immunosuppressive drugInternational Immunopharmacology, 2006
- Involvement of cyclooxygenase-2 in allergic nasal inflammation in ratsInternational Immunopharmacology, 2006
- Inflammation and cardiovascular disease mechanismsThe American Journal of Clinical Nutrition, 2006