Immuno-histochemical Evaluation of -methylacyl -CoA Racemase Expressionin Colorectal Carcinoma

Abstract

A case-control study aimed to evaluate the diagnostic value of Alpha-methylacyl-CoA racemase immunoreactivity in patients with colorectal neoplasia where paraff in blocks of colorectal malignant and normal tissue of patients were used. Histological f indings were compared with AMACR immunohistochemistry results. The in silico analysis was carried out to f ind the prognostic signif icance of AMACR expression levels in colon cancers. Atotal of 30 patients with colorectal cancer (46 biopsy specimens, 16 normal tissues and 30 cancerous tissues) were evaluated. The mean level of staining extent inpatientswas 87%(range 30 to100%). More than 90% of patients had a strong positive reaction and only 2 patients (6.7%) had a weakly response. The immunoreactivity in half of the cases was 2. There is no signif icant relationship between the type of cancer and gender. Similarly, the intensity and extent of staining in different types of colorectal cancer were not signif icantly different. In addition, the expression of theAMACR marker did not have a signif icant relationship with the age of the patients as well as the gender of the patients. Of the 16 normal colon samples, only 2 cases were weakly positive. Sensitivityand specif icitywere calculatedas 100% and 87 %, respectively. The upregulation of AMACR was signif icantly associated with a decreased survival rate. The findings acknowledge the potential capacity of the AMACR marker asadiagnostic tool in identified colon neoplasia.