Dendronized Gold Nanoparticles as Carriers for gp160 (HIV-1) Peptides: Biophysical Insight into Complex Formation
- 21 January 2021
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 37 (4), 1542-1550
- https://doi.org/10.1021/acs.langmuir.0c03159
Abstract
The unavailability of effective and safe human immunodeficiency virus (HIV) vaccines incites several approaches for development of the efficient antigen/adjuvant vaccination composite. In this study, three different dendronized gold nanoparticles (AuNPs 13–15) were investigated for a complexation ability with gp160 synthetic peptides derived from an HIV envelope. It has been shown that HIV peptides interacted with nanoparticles as evident from the changes in their secondary structures, restricted the mobility of the attached fluorescence dye, and enhanced peptide helicity confirmed by the fluorescence polarization and circular dichroism results. Transmission electron microscopy visualized complexes as cloud-like structures with attached nanoparticles. AuNP 13–15 nanoparticles bind negatively charged peptides depending on the number of functional groups; the fastest saturation and peptide retardation were observed for the most dendronized nanoparticle as indicated from dynamic light scattering, laser Doppler velocimetry, and agarose gel electrophoresis experiments. Dendronized gold nanoparticles can be considered one of the potential HIV peptide-based vaccination platforms.Keywords
Funding Information
- Narodowa Agencja Wymiany Akademickiej (Pl-SK 2019?2020, PPN/BIL/2018/1/0015)
- Comunidad de Madrid (IMMUNOTHERCAN-CM B2017/BMD-3733, NANODENDMED II-CM ref B2017/BMD-3703)
- Vedeck? Grantov? Agent?ra M?VVa? SR a SAV (VEGA 1/0756/20)
- Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina (CIBER-BBN)
- European Regional Development Fund (CIBER Actions, Iniciativa Ingenio 2010, Plan 2008-2011, the Consolider Program)
- Ministerio de Econom?a y Competitividad (CTQ2017-86224-P)
- European Cooperation in Science and Technology (COST CA17140)
- Instituto de Salud Carlos III (CIBER Actions, Consolider Program, Iniciativa Ingenio 2010, Plan 2008-2011)
- Junta de Comunidades de Castilla-La Mancha (SBPLY/17/180501/000358)
- Agent?ra na Podporu V?skumu a V?voja (APVV-14-0267, SK-BY-RD-19-0019, SK-PL-18-0080, SK-PL-2015-0021)
This publication has 35 references indexed in Scilit:
- High-density sub-100-nm peptide-gold nanoparticle complexes improve vaccine presentation by dendritic cells in vitroNanoscale Research Letters, 2013
- HIV reservoirsAIDS, 2012
- Carbosilane Dendrimers are a Non-Viral Delivery System for Antisense Oligonucleotides: Characterization of DendriplexesJournal of Biomedical Nanotechnology, 2012
- On the measurement of gold nanoparticle sizes by the dynamic light scattering methodColloid Journal, 2011
- Gel Electrophoresis of Gold-DNA NanoconjugatesJournal of Biomedicine and Biotechnology, 2007
- Induction of Structure and Function in a Designed Peptide upon Adsorption on a Silica NanoparticleAngewandte Chemie, 2006
- Using circular dichroism spectra to estimate protein secondary structureNature Protocols, 2006
- Recognition and Stabilization of Peptide α-Helices Using Templatable Nanoparticle ReceptorsJournal of the American Chemical Society, 2004
- Field-Induced Interfacial Properties of Gold Nanoparticles in AC Microelectrophoretic ExperimentsThe Journal of Physical Chemistry B, 2004
- Spectroscopic properties of fluorescein in living lymphocytesCytometry, 1987