Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using 1H‐NMR and LC–MS

Abstract

Semen descurainiae oil (SDO) is an important traditional Chinese medicine that was discovered to have the function of reducing blood lipid in recent years. Metabolomics approach of plasma, liver and kidney in rats were performed using ¹H‐NMR and LC‐MS to illuminate the lower blood lipid concentration effect of SDO, and the niacin was considered as active control. The measure of total cholesterol (TC) and low‐density lipoprotein cholesterol (LDL‐C) in plasma showed that SDO treatment decreased significantly the content of TC and LDL‐C. An orthogonal partial least squares‐discriminant analysis (OPLS‐DA) approach was applied to identify different metabolic profiles of plasma, liver and kidney in rats and to detect related potential biomarkers. The results suggested the metabolic profiles of control group (Con) and hyperlipidemia group (HLM) have significant difference and the SDO and niacin group (NA) have the effective anti‐hyperlipidemia function. The biomarkers primarily concern lipid metabolism, amino acid metabolism and glycometabolism, and the change in biomarkers indicated hyperlipidemia could cause the unbalance of these metabolic pathways in vivo and the SDO reduced blood lipid by repairing amino acid metabolism and lipid metabolism.