Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
Open Access
- 5 August 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Tropical Diseases
- Vol. 2, 707865
- https://doi.org/10.3389/fitd.2021.707865
Abstract
While molecular assays, such as RT-PCR, have been widely used throughout the COVID-19 pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing for RT-PCR has been implemented. However, pooling samples for antigen testing has not been evaluated. We propose a pooling strategy for antigen testing that would significantly expand SARS-CoV-2 surveillance, especially for low-to-middle income countries, schools, and workplaces. Our data demonstrate that combining of up to 20 nasal swab specimens per pool can expand surveillance with antigen tests, even if a pool contains only one positive sample.Keywords
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