Species-Specific Differences in C-5 Sterol Desaturase Function Influence the Outcome of Azole Antifungal Exposure
- 17 November 2021
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 65 (12)
- https://doi.org/10.1128/aac.01044-21
Abstract
The azole antifungals inhibit sterol 14α-demethylase (S14DM), leading to depletion of cellular ergosterol and the synthesis of an aberrant sterol diol that disrupts membrane function. In Candida albicans , sterol diol production is catalyzed by the C-5 sterol desaturase enzyme encoded by ERG3 .Funding Information
- HHS | NIH | National Institute of Allergy and Infectious Diseases (R33AI127607, R21AI156611)
This publication has 38 references indexed in Scilit:
- Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation in ERG11 and Exhibiting Cross-Resistance to Azoles and Amphotericin BAntimicrobial Agents and Chemotherapy, 2012
- Amino acid substitutions in the Candida albicans sterol 5,6-desaturase (Erg3p) confer azole resistance: characterization of two novel mutants with impaired virulenceJournal of Antimicrobial Chemotherapy, 2012
- Azole Resistance by Loss of Function of the Sterol Δ 5,6 -Desaturase Gene ( ERG3 ) in Candida albicans Does Not Necessarily Decrease VirulenceAntimicrobial Agents and Chemotherapy, 2012
- Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicansAntimicrobial Agents and Chemotherapy, 2010
- Sterol biosynthesis and sterol uptake in the fungal pathogen Pneumocystis cariniiFEMS Microbiology Letters, 2010
- Phylogeny.fr: robust phylogenetic analysis for the non-specialistNucleic Acids Research, 2008
- OPTIMIZER: a web server for optimizing the codon usage of DNA sequencesNucleic Acids Research, 2007
- Sterol uptake in Candida glabrata: Rescue of sterol auxotrophic strainsDiagnostic Microbiology and Infectious Disease, 2005
- The G464S Amino Acid Substitution in Candida albicans Sterol 14α-Demethylase Causes Fluconazole Resistance in the Clinic through Reduced AffinityBiochemical and Biophysical Research Communications, 1999
- Improved method for high efficiency transformation of intact yeast cellsNucleic Acids Research, 1992