COMPARATIVE ANALYSIS OF LIPID PEROXIDATION ACTIVITY IN SUSPENSIONS OF CRYOPRESERVED CORD BLOOD NUCLEAR CELLS UNDER EXPOSURE TO ANTIOXIDANTS - MEMBRANOPROTECTORS WITH DIFFERENT ACTION MECHANISMS

Abstract

An in-depth study of the oxidative homeostasis state into cell suspensions that contain hematopoietic stem cells is one of the key points for understanding ways to improve technologies for long-term storage of this material. Compounds with antioxidant action are considered promising additional cryoprotectants. Intensification of lipid peroxidation processes is one of the main factors causing disturbances in the barrier properties of cell membranes. Comparative analysis of changes in lipid peroxidation parameters during the cryopreservation-deconservation cycle showed that antioxidants-membrane protectors with different mechanisms of action (B-complex vitamins; α-lipoic acid, thiazotic acid morpholinium salt, 2-ethyl-6-methyl-3-hydroxy pyridine succinate) have similar features of a positive effect on the oxidative status of umbilical cord blood nuclear cell suspensions during cryopreservation. However, 2-ethyl-6-methyl-3-hydroxy pyridine succinate has a statistically significant advantage over α-lipoic acid and thiazotic acid morpholinium salt in terms of the conjugate formation dynamics during phospholipid peroxidation, which can be associated with its direct antioxidant effect.