Simultaneous Analysis of Bergapten and Schinifoline in Zanthoxylum schinifolium Seeds Using HPLC and UPLC-MS/MS Systems
Open Access
- 23 March 2023
- Vol. 12 (7), 1355
- https://doi.org/10.3390/foods12071355
Abstract
Zanthoxylum schinifolium Siebold et Zuccarini belongs to the Rutaceae family and has been widely used as a spice in East Asian countries such as Korea, China, and Japan. The present study focused on developing and validating a simultaneous analytical method for marker substances (bergapten and schinifoline) in Z. schinifolium seeds. This was achieved using high-performance liquid chromatography with a photo-diode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) systems. In the regression equation, all markers showed a coefficient of determination of ≥0.9990. Marker recovery was 96.90–105.16% (relative standard deviation (RSD) ≤ 2.23), and the intra- and interday precision was RSD < 3.00. Bergapten and schinifoline were detected in the seeds at 1.70–2.85 mg/g and 0.19–0.94 mg/g, respectively. This analytical method will improve quality control of Z. schinifolium seeds. Additionally, this assay will provide basic data and quality assurance for future biological activity experiments or clinical applications.Funding Information
- R&D Program for Forest Science Technology (2016101C10-1919-AB02, NAN1614220, D18260)
- Korea Institute of Oriental Medicine (KSN2022310, KSN1823311)
This publication has 17 references indexed in Scilit:
- Effects of Oils and Essential Oils from Seeds ofZanthoxylum schinifoliumagainst Foodborne Viral SurrogatesEvidence-Based Complementary and Alternative Medicine, 2014
- Radiosensitizing Effect of Schinifoline from Zanthoxylum schinifolium Sieb et Zucc on Human Non-Small Cell Lung Cancer A549 Cells: A Preliminary in Vitro InvestigationMolecules, 2014
- Chemical composition, antimicrobial and anti-inflammatory activities of the essential oil from Maqian (Zanthoxylum myriacanthum var. pubescens) in Xishuangbanna, SW ChinaJournal of Ethnopharmacology, 2014
- Analgesia Synergism of Essential Oil from Pericarp ofZanthoxylum schinifoliumand VerapamilEvidence-Based Complementary and Alternative Medicine, 2014
- Quantitative and qualitative analysis of common peaks in chemical fingerprint of Yuanhu Zhitong tablet by HPLC-DAD–MS/MSJournal of Pharmaceutical Analysis, 2014
- NF-κB Inhibitory Activities of Glycosides and Alkaloids from Zanthoxylum schinifolium StemsCHEMICAL & PHARMACEUTICAL BULLETIN, 2014
- Characterization and Comparison of the Pungent Components in Commercial Zanthoxylum bungeanum Oil and Zanthoxylum schinifolium OilJournal of Food Science, 2013
- Quinolone alkaloids with antibacterial and cytotoxic activities from the fruits of Evodia rutaecarpaFitoterapia, 2013
- Cytotoxic Constituents from the Leaves of Zanthoxylum schinifoliumBulletin of the Korean Chemical Society, 2010
- Glycoprotein of Zanthoxylum piperitum DC has a hepatoprotective effect via anti-oxidative character in vivo and in vitroToxicology in Vitro, 2008