Microparticle Responses to Aerobic Exercise and Meal Consumption in Healthy Men
- 26 March 2019
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Medicine & Science in Sports & Exercise
- Vol. 51 (9), 1935-1943
- https://doi.org/10.1249/mss.0000000000001985
Abstract
Microparticles (MPs) are shed extracellular vesicles that express the pro-thrombotic tissue factor (TF). Aerobic exercise may reduce MP count and TF expression. This study investigated the impact of acute running or rest followed by standardised meal consumption on MP phenotypes and TF expression. 15 males (age: 22.9 ± 3.3 years; body mass: 81.9 ± 11.4 kg; V[Combining Dot Above]O2 max 54.9 ± 6.5 mL·kg·min-1; mean ± SD) completed 1h of running (70% V[Combining Dot Above]O2max) or rest at 9am, and consumed a standardised meal (1170 kcal, 43% CHO, 17% PRO, 40% fat) at 10:45am. Venous blood samples were taken at 9am, 10am and 11:30am. MP concentration, diameter, phenotypes and TF-expression were assessed using nanoparticle tracking analysis (NTA) and flow cytometry. NTA identified no changes in MP concentration or diameter in response to time or trial. Flow cytometry revealed total MP count increased from 9am to 10am (1.62 ± 2.28 to 1.74 ± 2.61 x1010/L, p = .016, effect size (η2) = .105), but was unaffected by trial. TF+ platelet-derived MP % reduced from 9am to 10am (44.0 ± 21.2 to 21.5 ± 9.3%, p = .001, η2 = .582) after exercise only (control: 36.8 ± 18.2 to 34.9 ± 11.9%, p = .972). TF+ neutrophil-derived MP % reduced from 9am to 11:30am (42.3 ± 17.2 to 25.1 ± 14.9%, p = 0.048, η2 = .801) in the exercise trial only (control: 28.5 ± 15.7 to 32.2 ± 9.6%, p = .508). Running induced a significant reduction in %TF+ platelet and neutrophil MP, suggesting a transient reduction in cardiovascular risk via reduced TF-stimulated thrombosis. This requires further investigation over longer time periods in cardiovascular disease populations.This publication has 38 references indexed in Scilit:
- Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis PatientsPLOS ONE, 2013
- Microparticles in Health and DiseaseJournal of Veterinary Internal Medicine, 2013
- Circadian control of the immune systemNature Reviews Immunology, 2013
- Moderate-intensity, premeal cycling blunts postprandial increases in monocyte cell surface CD18 and CD11a and endothelial microparticles following a high-fat meal in young adultsApplied Physiology, Nutrition, and Metabolism, 2012
- Detection of circulating microparticles by flow cytometry: influence of centrifugation, filtration of buffer, and freezingVascular Health and Risk Management, 2010
- Release of pro-coagulant microparticles after moderate endurance exercisePlatelets, 2010
- The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein IbThrombosis and Haemostasis, 2010
- Microparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjectsThrombosis and Haemostasis, 2008
- Circadian rhythm of leucocytes and lymphocyte subsets and its possible correlation with the function of the autonomic nervous systemClinical and Experimental Immunology, 1997
- Influence of power and aerobic exercise training on haemostatic factors after coronary artery surgeryHeart, 1992