Assessment of TNF-α Gene Expression in Type 2 Diabetes Patients with Nephropathy
Open Access
- 31 December 2022
- journal article
- Published by CrossLinks International Publishers in Futuristic Biotechnology
Abstract
Type 2 diabetes mellitus (T2DM) and its complications, including nephropathy, are a significant public health concern worldwide. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays a crucial role in the development and progression of chronic inflammatory diseases, including T2DM and diabetic nephropathy. Objective: To evaluate of TNF-α gene expression in patients with T2DM and nephropathy compared to healthy individuals. Methods: The cross-sectional study conducted on 120 individuals divided into three groups: healthy individuals, type 2 diabetes mellitus (T2DM) patients without diabetic nephropathy, and T2DM patients with nephropathy. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The results showed almost 4.2-fold induced expression of TNF-α in T2DM patients without nephropathy compared to the normal group. Conclusion: The study reports that in diabetic nephropathy patients, Gene expression of gene TNF-a shows increases in cases when compared with healthy subjectsKeywords
This publication has 18 references indexed in Scilit:
- Association between TNF-α −308G/A polymorphism and diabetic nephropathy risk: a meta-analysisInternational Urology and Nephrology, 2013
- Interleukin-10 (-1082G/A) Gene Polymorphism in Patients With Type 2 Diabetes With and Without NephropathyGenetic Testing and Molecular Biomarkers, 2012
- Interleukin (IL)-10 Gene Polymorphisms Are Associated with Type 2 Diabetes With and Without Nephropathy: A Study of Patients from the Southeast Region of IranInflammation, 2011
- Connective Tissue Growth Factor in the Pathogenesis of Diabetic NephropathyPublished by Springer Science and Business Media LLC ,2007
- Association of lipoprotein lipase S447X, apolipoprotein E exon 4, and apoC3 −455T>C polymorphisms on the susceptibility to diabetic nephropathyClinical Genetics, 2006
- Renal Pro-Inflammatory Cytokine Gene Expression in Diabetic Nephropathy: Effect of Angiotensin-Converting Enzyme Inhibition and Pentoxifylline AdministrationAmerican Journal of Nephrology, 2006
- Development of Human Protein Reference Database as an Initial Platform for Approaching Systems Biology in HumansGenome Research, 2003
- Urinary and renal interstitial concentrations of TNF-α increase prior to the rise in albuminuria in diabetic ratsKidney International, 2003
- GENETICS OF NON-INSULIN-DEPENDENT (TYPE-II) DIABETES MELLITUSAnnual Review of Medicine, 1996
- The Stages in Diabetic Renal Disease: With Emphasis on the Stage of Incipient Diabetic NephropathyDiabetes, 1983