Development of PCR Detection System of Bacteriophages Pr 4 UGSHA, E7 ULSAU and Ye5 ULSAU

Abstract

Development of PCR detection system of bacteriophages Proteus phage (Pr (4)-UGSHA), Enterobacter phage (E-7-ULSAU) and Yersinia phage (Ye(5)-ULSAU) are presented. Phylogenetic tree of concordance of their genetic organization against each other was built which was between 24 to 31%. It was determined that specific fragment for Proteus phage (Pr (4)-UGSHA) is in the genome region of 3700-4500 bps. Highly specific fragments typical only for Enterobacter phage (E-7-ULSAU) and Yersinia phage Ye(5)ULSAU in studied genomes were not found, however, we didn't find regions during sharing of PCR that will allow detecting the only genome of a specific group. Two regions were established, their parallel determination was specific for Enterobacter phage and for Yersinia phage. In the BLAST system for parallel determination of genome fragments specific for groups of studied bacteriophages were determined. Finally, primer systems for PCR typing of bacteriophages were developed. They allowed to carry out identification of bacteriophages Proteus, Enterobacter and Yersinia, referring to specific groups in material obtained out of environment samples and pathological material without detachment of pure growth in screenings of specified groups during detection of genome fragment in 125 bps in size over the range of 3700-4500 bps of DNA Proteus phage (Pr (4)-UGSHA); sizes of 294 and 431 bps in size over the range of 17500-18000 and 26500-27500 bps of DNA Enterobacter phage (E-7-ULSAU) and 226 and 85 bps in size over the range of 2000-2500 and 24100-24300 bps of Yersinia phage (Ye(5)-ULSAU).