The objective was to analyze the effect of the type of semen, conventional and sexed, on the in vitro production of embryos. A total of 1,285 oocytes and 1,155 oocytes with sexed semen were matured and fertilized with conventional semen, obtaining 1,167 (90.55%) and 1,051 (90.68%) zygotes in fertilization with conventional and sexed semen, respectively (P=0.8411). In in vitro culture, embryo cleavage was evaluated in 769 (67.42%) of zygotes from conventional semen and 540 (55.12%) from zygotes from sexed semen (P=0.8411). There was no statistical difference in both analyzed variables. On day 7, the number of viable embryos produced was evaluated, being 428 (53.55%) conventional embryos and 138 (30.64%) viable embryos from sexed semen (P=0.0001). There was a significant difference, therefore, it obtained better embryonic conversion with conventional semen in relation to sexed semen of Nelore bulls.