Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions
Open Access
- 31 March 2011
- journal article
- Published by European Journal of Chemistry in European Journal of Chemistry
- Vol. 2 (1), 109-112
- https://doi.org/10.5155/eurjchem.2.1.109-112.147
Abstract
Cyclic voltammetric behaviour of three flavonoids: quercetin, morin and rutin was investigated for their interaction with DNA at pH = 4.7 and pH = 7.4 at body temperature (310 K), using glassy carbon electrode. The diffusion coefficients of the free and DNA bound forms of the flavonoids were evaluated using Randles–Sevcik equation. The binding parameters like binding constant, binding site size and binding free energy were also determined from voltammetric data. Moreover, the binding modes of flavonoids with DNA were evaluated from viscommetric analysis. A comparatively high value of binding constant, binding site size and diffusion coefficients for quercetin reveals its stronger binding with DNA under physiological conditions. The negative values of ∆G indicate the spontaneity of binding of flavonoids with DNA.Keywords
This publication has 16 references indexed in Scilit:
- Electrochemical and Spectroscopic Investigations of Protonated Ferrocene-DNA IntercalationAnalytical Sciences, 2008
- The Central Role of Metal Coordination in Selenium Antioxidant ActivityInorganic Chemistry, 2005
- Flavonoids: Promising anticancer agentsMedicinal Research Reviews, 2003
- Electrochemical studies of quercetin interacting with DNAMicrochemical Journal, 2002
- Flavonoids as AntioxidantsJournal of Natural Products, 2000
- DNA Cleavage by a Chromium(III) ComplexBiochemical and Biophysical Research Communications, 2000
- Bioavailability and metabolism of the flavonol quercetin in the pigFree Radical Biology & Medicine, 2000
- Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectorsCell Biology and Toxicology, 2000
- Dietary Flavonoids: Intake, Health Effects and BioavailabilityFood and Chemical Toxicology, 1999
- Studies of interaction between Safranine T and double helix DNA by spectral methodsSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 1998