Amelioration of Scopolamine-Induced Amnesic, Anxiolytic and Antidepressant Effects of Ficus benghalensis in Behavioral Experimental Models
Open Access
- 23 March 2020
- Vol. 56 (3), 144
- https://doi.org/10.3390/medicina56030144
Abstract
Background and Objectives: Ficus benghalensis (FB) is a commonly found tree in Pakistan and its various parts have folkloric importance in managing neurological ailments. In the present study, methanolic extract of its bark has been tested on an experimental animal model to evaluate memory-enhancing, anxiolytic and antidepressant activities to validate the claimed therapeutic potential. Materials and Methods: Methanolic extract of freshly isolated bark was prepared and subjected to preliminary phytochemical studies and gas chromatography–mass spectrometry (GC–MS) analysis for the presence of phytocomponents. To evaluate its effect on spatial learning, passive-avoidance test–step through (PAT-ST), Y-maze and Morris water maze (MWM) tests were carried out. Open-field (OFT) and elevated plus maze (EPM) tests were employed to explore the anti-anxiety potential of FB while a forced swimming test (FST) was utilized to assess its anti-depressant prospective. FB doses of 100, 200 and 300 mg/kg with positive and negative controls given to Sprague Dawley (SD) rats. Results: phytochemical studies showed the presence of various phytoconstituents including alkaloids, flavonoids, terpenes, phenolics and anthraquinones. The presence of synephrine, aspargine, glucose, fructose and fatty acids was revealed by GC–MS analysis. FB administration led to significant improved memory retention when evaluated through passive avoidance (p < 0.05), Y-maze (p < 0.05) and Morris water maze (p < 0.05) tests in a scopolamine model of amnesic rats. When tested by open field and elevated plus maze tests, FB demonstrated anxiety-resolving characteristics (p < 0.05) as animals dared to stay in open areas more than a control group. Mobility time was increased and immobility time was reduced (p < 0.05–0.01) in rats treated with FB, unveiling the anti-depressant importance of F. benghalensis. Conclusion: methanolic extract of F. benghalensis bark furnished scientific proof behind folkloric claims of the memory improving, anxiety-reducing and depression-resolving characteristics of the plant. These activities might be possible due to interaction of its phytoconstituents with serotonergic, glutamatergic, cholinergic and GABAergic systems in the brain.Keywords
This publication has 70 references indexed in Scilit:
- The Role of Dietary Polyphenols on Adult Hippocampal Neurogenesis: Molecular Mechanisms and Behavioural Effects on Depression and AnxietyOxidative Medicine and Cellular Longevity, 2012
- Butyrylcholinesterase Is Associated With β-Amyloid Plaques in the Transgenic APPSWE/PSEN1dE9 Mouse Model of Alzheimer DiseaseJournal of Neuropathology and Experimental Neurology, 2012
- Pathogenic protein seeding in alzheimer disease and other neurodegenerative disordersAnnals of Neurology, 2011
- The cholinergic system and spatial learningBehavioural Brain Research, 2011
- Flavonoid modulation of GABAA receptorsBritish Journal of Pharmacology, 2011
- A Molecular Basis for Interactions Between Sleep and MemorySleep Medicine Clinics, 2011
- Flavonoids and brain health: multiple effects underpinned by common mechanismsGenes & Nutrition, 2009
- Prevalence of stress, anxiety and depression in with Alzheimer caregiversHealth and Quality of Life Outcomes, 2008
- The use of the elevated plus maze as an assay of anxiety-related behavior in rodentsNature Protocols, 2007
- Morris water maze: procedures for assessing spatial and related forms of learning and memoryNature Protocols, 2006