Back from the dead; the curious tale of the predatory cyanobacteriumVampirovibrio chlorellavorus
Open Access
- 21 May 2015
- Vol. 3, e968
- https://doi.org/10.7717/peerj.968
Abstract
An uncultured non-photosynthetic basal lineage of the Cyanobacteria, the Melainabacteria, was recently characterised by metagenomic analyses of aphotic environmental samples. However, a predatory bacterium, Vampirovibrio chlorellavorus, originally described in 1972 appears to be the first cultured representative of the Melainabacteria based on a 16S rRNA sequence recovered from a lyophilised co-culture of the organism. Here, we sequenced the genome of V. chlorellavorus directly from 36 year-old lyophilised material that could not be resuscitated confirming its identity as a member of the Melainabacteria. We identified attributes in the genome that likely allow V. chlorellavorus to function as an obligate predator of the microalga Chlorella vulgaris, and predict that it is the first described predator to use an Agrobacterium tumefaciens-like conjugative type IV secretion system to invade its host. V. chlorellavorus is the first cyanobacterium recognised to have a predatory lifestyle and further supports the assertion that Melainabacteria are non-photosynthetic.This publication has 89 references indexed in Scilit:
- Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolutionProceedings of the National Academy of Sciences of the United States of America, 2012
- Gene and translation initiation site prediction in metagenomic sequencesBioinformatics, 2012
- An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaeaThe ISME Journal, 2011
- IMG ER: a system for microbial genome annotation expert review and curationBioinformatics, 2009
- FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance MatrixMolecular Biology and Evolution, 2009
- IslandViewer: an integrated interface for computational identification and visualization of genomic islandsBioinformatics, 2009
- UniRef: comprehensive and non-redundant UniProt reference clustersBioinformatics, 2007
- RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed modelsBioinformatics, 2006
- Making sense of it all: bacterial chemotaxisNature Reviews Molecular Cell Biology, 2004
- The versatile bacterial type IV secretion systemsNature Reviews Microbiology, 2003