Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field
Open Access
- 8 December 2022
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 12 (12), 1146
- https://doi.org/10.3390/bios12121146
Abstract
Porcine epidemic diarrhea virus (PEDV), a coronavirus that causes highly infectious intestinal diarrhea in piglets, has led to severe economic losses worldwide. Rapid diagnosis and timely supervision are significant in the prophylaxis of PEDV. Herein, we proposed a gold-nanorod (GNR) probe-assisted counting method using dark field microscopy (DFM). The antibody-functionalized silicon chips were prepared to capture PEDV to form sandwich structures with GNR probes for imaging under DFM. Results show that our DFM-based assay for PEDV has a sensitivity of 23.80 copies/μL for simulated real samples, which is very close to that of qPCR in this study. This method of GNR probes combined with DFM for quantitative detection of PEDV not only has strong specificity, good repeatability, and a low detection limit, but it also can be implemented for rapid on-site detection of the pathogens.Keywords
Funding Information
- National Natural Science Foundation of China (31870989)
- Shanghai Science and Technology Innovation Action Plan in 2022 (22N31900800)
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- The Hospital Research Foundation Group
This publication has 30 references indexed in Scilit:
- The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea VirusScientific Reports, 2016
- Porcine epidemic diarrhea virus: An overview of current virological and serological diagnostic methodsVirus Research, 2016
- Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virusVirology Journal, 2015
- Biosensing with plasmonic nanosensorsNature Materials, 2008
- Noble Metals on the Nanoscale: Optical and Photothermal Properties and Some Applications in Imaging, Sensing, Biology, and MedicineAccounts of Chemical Research, 2008
- A One-Step Homogeneous Immunoassay for Cancer Biomarker Detection Using Gold Nanoparticle Probes Coupled with Dynamic Light ScatteringJournal of the American Chemical Society, 2008
- Drastic Reduction of Plasmon Damping in Gold NanorodsPhysical Review Letters, 2002
- Single-target molecule detection with nonbleaching multicolor optical immunolabelsProceedings of the National Academy of Sciences of the United States of America, 2000
- Immobilization of ultra-thin layer of monoclonal antibody on glass surfaceJournal of Chromatography B: Biomedical Sciences and Applications, 1991
- [49] Preparation of polyclonal antibodiesMethods in Enzymology, 1990