Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KTpClPB

Abstract

Potentiometric sensors provide an opportunity to perform biomedical, environmental analysis, and industry analysis, these sensors are generally easy to use, portable, simple, and inexpensive. The potentiometric method is a method of measuring the potential difference of the balance between the indicator electrode and the reference electrode. Chemical sensors are a device that can convert chemical quantities into electrical quantities. Chemical sensors sensitive to one analyte involve a combination of molecular recognition elements PVA-enzyme PVC-KTpClPB and transducer to detect an analytical bond through a voltage signal. The urease enzyme is used for the determination of urea, the urea sensor means an accurate determination of urea essential with respect to renal function and biomedical applications. ISE is a good approach to build chemical sensors in detecting urea analyte. The immobilized product as a molecular recognition element of PVC-enzyme PVC-KTpClPB has a sensitivity of 19,069 mV/decade R² = 0.94 with a range of 1.10-5 - 5.10-4 M in electrolyte solution consisting of buffer KH₂PO₄ 0.001 M and KCl 0.001 M.