Raman spectroscopy is a technique based on inelastic scattering of molecular systems when illuminated by monochromatic radiation. Owing to its high chemical specificity and noninvasive detection capability, in the last decade, Raman scattering has found wide application in cancer screening and diagnosis. In this paper; we describe recent results obtained by applying Raman spectroscopy to osteosarcoma and chondrosarcoma; the two main bone tumors. The results show the remarkable potential of Thermo Scientific™ DXR™2 microscope to discriminate between subcellular components inside osteo-differentiated osteoblasts and osteosarcoma cells or; at level of tissues; to discriminate chondrogenic tumors giving the possibility to grade the level of malignancy the cartilaginous tumors under investigation.