Drosophila telomere capping protein HOAP interacts with DSB sensor proteins Mre11 and Nbs
- 8 February 2021
- journal article
- research article
- Published by Wiley in Genes to Cells
- Vol. 26 (4), 219-229
- https://doi.org/10.1111/gtc.12836
Abstract
In eukaryotes, specific DNA‐protein structures called telomeres exist at linear chromosome ends. Telomere stability is maintained by a specific capping protein complex. This capping complex is essential for the inhibition of the DNA damage response (DDR) at telomeres and contributes to genome integrity. In Drosophila the central factors of telomere capping complex are HOAP and HipHop. Furthermore, a DDR protein complex Mre11‐Rad50‐Nbs (MRN) is known to be important for the telomere association of HOAP and HipHop. However, whether MRN interacts with HOAP and HipHop, and the telomere recognition mechanisms of HOAP and HipHop are poorly understood. Here, we show that Nbs interacts with Mre11 and transports the Mre11‐Rad50 complex from the cytoplasm to the nucleus. In addition, we report that HOAP interacts with both Mre11 and Nbs. The N‐terminal region of HOAP is essential for its co‐localization with HipHop. Finally, we reveal that Nbs interacts with the N‐terminal region of HOAP.Keywords
This publication has 43 references indexed in Scilit:
- NBS1 interacts with HP1 to ensure genome integrityCell Death & Disease, 2019
- Drosophila: Retrotransposons Making up TelomeresViruses, 2017
- Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cellsNucleic Acids Research, 2014
- Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9Biology Open, 2013
- Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and agingNature Medicine, 2006
- Drosophila ATM and ATR checkpoint kinases control partially redundant pathways for telomere maintenanceProceedings of the National Academy of Sciences of the United States of America, 2005
- The Drosophila Mre11/Rad50 Complex Is Required to Prevent Both Telomeric Fusion and Chromosome BreakageCurrent Biology, 2004
- Novel Drosophila Heterochromatin Protein 1 (HP1)/Origin Recognition Complex-associated Protein (HOAP) Repeat Motif in HP1/HOAP Interactions and Chromocenter AssociationsOnline Journal of Public Health Informatics, 2003
- The Drosophila HOAP protein is required for telomere cappingNature, 2002
- The hMre11/hRad50 Protein Complex and Nijmegen Breakage Syndrome: Linkage of Double-Strand Break Repair to the Cellular DNA Damage ResponseCell, 1998