Unraveling the plant diversity of the Amazonian canga through DNA barcoding
Open Access
- 31 August 2021
- journal article
- research article
- Published by Wiley in Ecology and Evolution
- Vol. 11 (19), 13348-13362
- https://doi.org/10.1002/ece3.8057
Abstract
The canga of the Serra dos Carajás, in Eastern Amazon, is home to a unique open plant community, harboring several endemic and rare species. Although a complete flora survey has been recently published, scarce to no genetic information is available for most plant species of the ironstone outcrops of the Serra dos Carajás. In this scenario, DNA barcoding appears as a fast and effective approach to assess the genetic diversity of the Serra dos Carajás flora, considering the growing need for robust biodiversity conservation planning in such an area with industrial mining activities. Thus, after testing eight different DNA barcode markers (matK, rbcL, rpoB, rpoC1, atpF-atpH, psbK-psbI, trnH-psbA, and ITS2), we chose rbcL and ITS2 as the most suitable markers for a broad application in the regional flora. Here we describe DNA barcodes for 1,130 specimens of 538 species, 323 genera, and 115 families of vascular plants from a highly diverse flora in the Amazon basin, with a total of 344 species being barcoded for the first time. In addition, we assessed the potential of using DNA metabarcoding of bulk samples for surveying plant diversity in the canga. Upon achieving the first comprehensive DNA barcoding effort directed to a complete flora in the Brazilian Amazon, we discuss the relevance of our results to guide future conservation measures in the Serra dos Carajás.Funding Information
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (307479/2016‐1, 402756/2018‐5, 444227/2018‐0)
This publication has 79 references indexed in Scilit:
- phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census DataPLOS ONE, 2013
- How Effective Are DNA Barcodes in the Identification of African Rainforest Trees?PLOS ONE, 2013
- MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and UsabilityMolecular Biology and Evolution, 2013
- Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for FungiProceedings of the National Academy of Sciences of the United States of America, 2012
- Identification of the vascular plants of Churchill, Manitoba, using a DNA barcode libraryBMC Ecology, 2012
- Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in PanamaProceedings of the National Academy of Sciences of the United States of America, 2009
- Identification of Amazonian Trees with DNA BarcodesPLOS ONE, 2009
- Genome-wide association analysis reveals a SOD1 mutation in canine degenerative myelopathy that resembles amyotrophic lateral sclerosisProceedings of the National Academy of Sciences of the United States of America, 2009
- Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally WellPLOS ONE, 2008
- DNA barcoding the floras of biodiversity hotspotsProceedings of the National Academy of Sciences of the United States of America, 2008