Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons
Open Access
- 18 May 2020
- journal article
- Published by F1000 Research Ltd in AMRC Open Research
Abstract
Good's buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced pluripotent stem cell-derived neurons, this study explores the effect of zwitterionic buffers, specifically HEPES, on lysosomal volume and Ca2+ levels. Certain zwitterionic buffers lead to lysosomal expansion and reduced lysosomal Ca2+. Care should be taken when selecting buffers for growth media to avoid detrimental impacts on lysosomal function.Keywords
Funding Information
- Medical Research Council (1976191)
- March of Dimes Foundation (#5-FY12-117)
- Life Sciences Research Network Wales (NRN511389)
- Batten Disease Family Association
- Royal Society (RG110215)
- Action Medical Research (GN2069)
- Alzheimer’s Research UK (ARUK-IRG2015-7)
- Wellcome Trust
- Niemann-Pick Disease Group UK
- Medical Research Council (MR/P007651/1)
This publication has 18 references indexed in Scilit:
- Forced cell cycle exit and modulation of GABAA, CREB, and GSK3β signaling promote functional maturation of induced pluripotent stem cell-derived neuronsAmerican Journal of Physiology-Cell Physiology, 2016
- Presenilin 1 Maintains Lysosomal Ca2+ Homeostasis via TRPML1 by Regulating vATPase-Mediated Lysosome AcidificationCell Reports, 2015
- “Zwitterionic Proton Sponge” Hydrogen Bonding Investigations on the Basis of Car–Parrinello Molecular DynamicsJournal of Chemical Information and Modeling, 2015
- Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixationBMC Developmental Biology, 2015
- A rapid method for the preparation of ultrapure, functional lysosomes using functionalized superparamagnetic iron oxide nanoparticlesPublished by Elsevier BV ,2015
- Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calciumNature Medicine, 2008
- Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA PolyplexesOnline Journal of Public Health Informatics, 2003
- Niemann-Pick Type C1 (NPC1) Overexpression Alters Cellular Cholesterol HomeostasisOnline Journal of Public Health Informatics, 2000
- Hydrogen Ion Buffers for Biological Research*Biochemistry, 1966
- Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissueBiochemical Journal, 1955