Micromorphological differentiation of Korean Disporum species using light and scanning electron microscopy
- 10 May 2021
- journal article
- research article
- Published by Wiley in Microscopy Research and Technique
- Vol. 84 (11), 2614-2624
- https://doi.org/10.1002/jemt.23812
Abstract
The genus Disporum Salisb. is widely distributed in East Asia, yet phylogenetically relevant morphological traits useful for differentiating many of the small, perennial, herbaceous species remain poorly described. To address this, leaf, floral, pollen, and orbicule micromorphology of four Korean Disporum species was investigated using light and scanning electron microscopy. All Korean Disporum species examined had hypostomatic leaves, with anomocytic stomatal complexes found only on the abaxial epidermis. Guard cell length varied among species, ranging from 44.30 μm in D. viridescens to 53.49 μm in D. uniflorum. The epidermal cells of the investigated Disporum taxa had sinuate anticlinal cell walls on both adaxial and abaxial surfaces. The surface of the guard and subsidiary cells were either smooth with weak striations or had strongly wrinkled striations. The pollen grains of all Korean Disporum taxa were monads, monosulcate with granular aperture membranes, subprolate to prolate in shape with microreticulate or verrucate exine surfaces. The mean size of pollen grains ranged from 46.38 to 49.92 μm in polar length and from 34.39 to 39.58 μm in equatorial diameter across species. Sexine ornamentation was a taxonomically relevant trait for differentiating Korean Disporum taxa. Additionally, the presence of orbicules as well as the orbicular characters (e.g., size, shape, ornamentation, and association pattern) are described for the first time in species from this genus. The present investigation of leaf and floral micromorphology using light and scanning electron microscopy provides valuable information for the taxonomic differentiation and identification of Disporum species in Korea. Research Highlights A detailed micromorphological description of leaf, floral characters (tepal, stigma, style), pollen and orbicule is provided for Korean Disporum species using scanning electron microscopy (SEM) and light microscopy (LM). The presence of orbicules and their taxonomic implications in Korean Disporum species are described for the first time. Phylogenetically informative pollen and orbicule micromorphological characters are described, improving understanding the systematic relationships of Korean species in the genus Disproum.Keywords
This publication has 56 references indexed in Scilit:
- Distribution of orbicules in Annonaceae mirrors evolutionary trend in angiospermsPlant Ecology and Evolution, 2010
- Floral nectary structure, nectar production, and carbohydrate composition in theLiliumAsiatic hybrid ‘Trésor’Botany, 2010
- Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae)Botanical Journal of the Linnean Society, 2009
- Disporum jinfoshanense X. Z. Li, D. M. Zhang D. Y. Hong, a new species of the Liliaceae from Chongqing, ChinaActa Phytotaxonomica Sinica, 2007
- Morphology of pollen and orbicules in some Dioscorea species and its systematic implicationsBotanical Journal of the Linnean Society, 2001
- Morphology of pollen and orbicules in some Dioscorea species and its systematic implicationsBotanical Journal of the Linnean Society, 2001
- Recognition of the New World Disporum section Prosartes as Prosartes (Liliaceae) based on the sequence data of the rbcL geneTaxon, 1994
- Molecular Systematics of Liliaceae — Asparagoideae — Polygonatae. I. RFLP Analysis of cpDNA in Several Asiatic Disporum SpeciesPlant Species Biology, 1994
- Biosystematic Studies in Disporum (Liliaceae‐Polygonateae) IV. Karyotype Analysis of some Asiatic and North American Taxa with Special Reference to their Systematic Status1)Plant Species Biology, 1992
- Pollen Morphology and Taxonomy of the Genus Quararibea S.l. (Bombacaceae)Bulletin du Jardin botanique national de Belgique / Bulletin van de National Plantentuin van België, 1974