Label-Free Luminescent Switch-On Probe for Ochratoxin A Detection Using a G-Quadruplex-Selective Iridium(III) Complex

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Abstract
A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R-2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.
Funding Information
  • Agence Nationale de la Recherche (A-HKBU201/12)
  • Universidade de Macau (MRG007/LCH/2014/ICMS, MRG023/LCH/2013/ICMS, MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH)
  • Health and Medical Research Fund (HMRF/13121482, HMRF/14130522)
  • Research Grants Council, University Grants Committee, Hong Kong (A-HKBU201/12, HKBU/201811, HKBU/201913, HKBU/204612)
  • Hong Kong Baptist University (FRG2/13-14/008, FRG2/14-15/004)
  • Science and Technology Development Fund of Macao Special Administrative Region (103/2012/A3)
  • Shum Yiu Foon Sum Bik Chuen Memorial Centre for Cancer and Inflammation Research, Hong Kong Baptist University
  • State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University (SKLP-14-15-P001)