Comparison of lipidome profiles of Caenorhabditis elegans—results from an inter-laboratory ring trial
Open Access
- 17 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Metabolomics
- Vol. 17 (3), 1-14
- https://doi.org/10.1007/s11306-021-01775-6
Abstract
Introduction Lipidomic profiling allows 100s if not 1000s of lipids in a sample to be detected and quantified. Modern lipidomics techniques are ultra-sensitive assays that enable the discovery of novel biomarkers in a variety of fields and provide new insight in mechanistic investigations. Despite much progress in lipidomics, there remains, as for all high throughput “omics” strategies, the need to develop strategies to standardize and integrate quality control into studies in order to enhance robustness, reproducibility, and usability of studies within specific fields and beyond. Objectives We aimed to understand how much results from lipid profiling in the model organism Caenorhabditis elegans are influenced by different culture conditions in different laboratories. Methods In this work we have undertaken an inter-laboratory study, comparing the lipid profiles of N2 wild type C. elegans and daf-2(e1370) mutants lacking a functional insulin receptor. Sample were collected from worms grown in four separate laboratories under standardized growth conditions. We used an UPLC-UHR-ToF–MS system allowing chromatographic separation before MS analysis. Results We found common qualitative changes in several marker lipids in samples from the individual laboratories. On the other hand, even in this controlled experimental system, the exact fold-changes for each marker varied between laboratories. Conclusion Our results thus reveal a serious limitation to the reproducibility of current lipid profiling experiments and reveal challenges to the integration of such data from different laboratories.Funding Information
- Projekt DEAL
This publication has 34 references indexed in Scilit:
- Functional Loss of Two Ceramide Synthases Elicits Autophagy-Dependent Lifespan Extension in C. elegansPLOS ONE, 2013
- A study of Caenorhabditis elegans DAF-2 mutants by metabolomics and differential correlation networksMolecular BioSystems, 2013
- Batch Normalizer: A Fast Total Abundance Regression Calibration Method to Simultaneously Adjust Batch and Injection Order Effects in Liquid Chromatography/Time-of-Flight Mass Spectrometry-Based Metabolomics Data and Comparison with Current Calibration MethodsAnalytical Chemistry, 2012
- Excessive folate synthesis limits lifespan in the C. elegans: E. coliaging modelBMC Biology, 2012
- The fatty acid synthasefasn-1acts upstream of WNK and Ste20/GCK-VI kinases to modulate antimicrobial peptide expression inC. elegansepidermisVirulence, 2010
- The Influence of Bacterial Diet on Fat Storage in C. elegansPLOS ONE, 2009
- A branched-chain fatty acid is involved in post-embryonic growth control in parallel to the insulin receptor pathway and its biosynthesis is feedback-regulated in C. elegansJournal of Bone and Joint Surgery, 2008
- Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomicsJournal of Lipid Research, 2008
- The emerging field of lipidomicsNature Reviews Drug Discovery, 2005
- A Palmitoyl-CoA-Specific Δ9 Fatty Acid Desaturase from Caenorhabditis elegansBiochemical and Biophysical Research Communications, 2000