Analysis of Associative Relationship of Allelic and Genotypical Variants of Polymorphism Rs 2010963 of the VEGFA Gene with Formation and Development of Ronchopathy

Abstract

It is interesting to note that the adverse effect of this genotype was observed exclusively in patients with ronchopathy, while in patients with ronchopathy, the frequency of this genotype did not differ in comparison with the control group, i.e. there is a significant tendency to an increase in the genotype with an increase in the severity of the pathology. Material and methods. To solve the set tasks, 208 patients with various diseases of the upper respiratory tract, with nasal breathing disorders, causing ronchopathy, who were hospitalized in the ENT department of the multidisciplinary clinic of the Tashkent Medical Academy for 2015 to 2021, were examined. The control group consisted of 50 apparently healthy people who agreed to participate in the study (students, masters, clinical residents). Among the sick men there were 144 (73%), women – 64 (27%). The age of the patients ranged from 18 to 70 years, averaging 44.5 ± 6.8 years. Molecular genetic studies were carried out in the Department of Molecular Medicine and Cell Technologies of the RSNPMC Hematology. This part of the work consisted of several stages: 1. Blood sampling. 2. Isolation of DNA from peripheral blood lymphocytes. 3. Carrying out PCR. 4. Conducting electrophoresis and visualizing the results (if necessary). The analysis of the TGFb1 gene polymorphism associations was carried out using a case-control model (case-control, comparison of two samples). The sample “case” was formed from 104 patients with ronchopathy. Conclusion. Since this work is one of the few works on the study of the relationship between rs 2010963 of the VEGFA gene and the risk of developing ronchopathy, our data may become the subject of further discussions.