Can Interspecies Hybrid Zygosaccharomyces rouxii Produce an Allohaploid Gamete?
Open Access
- 1 January 2018
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 84 (1), e01845-17
- https://doi.org/10.1128/aem.01845-17
Abstract
In soy sauce manufacturing, Candida versatilis plays a role in the production of volatile flavor compounds, such as volatile phenols, but limited accessible information on its genome has prevented further investigation regarding aroma production and breeding. Although the draft genome sequence data of two strains of C. versatilis have recently been reported, these strains are not similar to each other. Here, we reassess the draft genome sequence data for strain t-1, which was originally reported to be C. versatilis, and conclude that strain t-1 is most probably not C. versatilis but a gamete of hybrid Zygosaccharomyces rouxii. Phylogenetic analysis of the D1/D2 region of the 26S ribosomal DNA (rDNA) sequence indicated that strain t-1 is more similar to the genus Zygosaccharomyces than to C. versatilis. Moreover, we found that the genome of strain t-1 is composed of haploid genome content and divided into two regions that show approximately 100% identity with the T or P subgenome derived from the natural hybrid Zygosaccharomyces rouxii, such as NBRC110957 and NBRC1876. We also found a chromosome crossing-over signature in the scaffolds of strain t-1. These results suggest that strain t-1 is a gamete of the hybrid Z. rouxii, generated by either meiosis or chromosome loss following reciprocal translocation between the T and P subgenomes. Although it is unclear why strain t-1 was misidentified as C. versatilis, the genome of strain t-1 has broad implications for considering the evolutionary fate of an allodiploid.Keywords
This publication has 39 references indexed in Scilit:
- Diversity of Mating-Type Chromosome Structures in the Yeast Zygosaccharomyces rouxii Caused by Ectopic Exchanges between MAT-Like LociPLOS ONE, 2013
- Recurrent Rearrangement during Adaptive Evolution in an Interspecific Yeast Hybrid Suggests a Model for Rapid IntrogressionPLoS Genetics, 2013
- A pipeline for automated annotation of yeast genome sequences by a conserved-synteny approachBMC Bioinformatics, 2012
- Different selective pressures lead to different genomic outcomes as newly-formed hybrid yeasts evolveBMC Evolutionary Biology, 2012
- Multiple Molecular Mechanisms Cause Reproductive Isolation between Three Yeast SpeciesPLoS Biology, 2010
- Altered Heterochromatin Binding by a Hybrid Sterility Protein in Drosophila Sibling SpeciesScience, 2009
- A Screen for Recessive Speciation Genes Expressed in the Gametes of F1 Hybrid YeastPLoS Genetics, 2007
- Sequence Diversity, Reproductive Isolation and Species Concepts in SaccharomycesGenetics, 2006
- The Yeast Gene Order Browser: Combining curated homology and syntenic context reveals gene fate in polyploid speciesGenome Research, 2005
- Basic local alignment search toolJournal of Molecular Biology, 1990