Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

Abstract

A simple, sensitive, accurate and cost-effective method has been described for the quantitative determination of Tetracycline hydrochloride (TCs) in bulk drug and its pharmaceutical preparations in an aqueous medium. The proposed method is based on the nucleophilic substitution reaction of 9-chloroacridine (9-CA) as a chromogenic reagent with TCs in an aqueous solution forming a colored complex. The spectrum of the product shows maximum absorption at 385 nm against the blank. The linearity range of the calibration graph was (1-28) μg.mL-1 with a limited detection value (LOD) 0.138 μg.mL-1. The results show the absence of interferences from the excipients compounds on the determination of the drug. The suggested method exhibited high reproducibility (RSD) 2.3 % with mean percentage recovery (R%) 99.95. Furthermore, the stability constant has been calculated to be 9.843×105 L.mol-1 and the reaction mechanism is proposed. A ratio of 1: 1 was found between the above drug and the reagent using Job and molar ratio methods. The accuracy and validity of the proposed method were further determined by performing a recovery experiment by the use of the standard addition method. It is found that the method does not require extraction process and it agree well standard addition method. Appli