Association between algal productivity and phycosphere composition in an outdoor Chlorella sorokiniana reactor based on multiple longitudinal analyses
Open Access
- 25 May 2020
- journal article
- research article
- Published by Wiley in Microbial Biotechnology
- Vol. 13 (5), 1546-1561
- https://doi.org/10.1111/1751-7915.13591
Abstract
Microalgae as a biofuel source are of great interest. Bacterial phycosphere inhabitants of algal cultures are hypothesized to contribute to productivity. In this study, the bacterial composition of the Chlorella sorokiniana phycosphere was determined over several production cycles in different growing seasons by 16S rRNA gene sequencing and identification. The diversity of the phycosphere increased with time during each individual reactor run, based on Faith’s phylogenetic diversity metric versus days post-inoculation (R = 0.66, P < 0.001). During summer months, Vampirovibrio chlorellavorus, an obligate predatory bacterium, was prevalent. Bacterial sequences assigned to the Rhizobiales, Betaproteobacteriales and Chitinophagales were positively associated with algal biomass productivity. Applications of the general biocide, benzalkonium chloride, to a subset of experiments intended to abate V. chlorellavorus appeared to temporarily suppress phycosphere bacterial growth, however, there was no relationship between those bacterial taxa suppressed by benzalkonium chloride and their association with algal productivity, based on multinomial model correlations. Algal health was approximated using a model-based metric, or the ‘Health Index’ that indicated a robust, positive relationship between C. sorokiniana fitness and presence of members belonging to the Burholderiaceae and Allorhizobium–Neorhizobium–Pararhizobium–Rhizobium clade. Bacterial community composition was linked to the efficiency of microalgal biomass production and algal health.Keywords
Funding Information
- U.S. Department of Energy (DE‐EE0006269)
This publication has 75 references indexed in Scilit:
- MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and UsabilityMolecular Biology and Evolution, 2013
- Growing Unculturable BacteriaJournal of Bacteriology, 2012
- Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platformsThe ISME Journal, 2012
- Dirichlet Multinomial Mixtures: Generative Models for Microbial MetagenomicsPLOS ONE, 2012
- Variance adjusted weighted UniFrac: a powerful beta diversity measure for comparing communities based on phylogenyBMC Bioinformatics, 2011
- The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic SexTHE PLANT CELL ONLINE, 2010
- FastTree 2 – Approximately Maximum-Likelihood Trees for Large AlignmentsPLOS ONE, 2010
- Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advancesThe Plant Journal, 2008
- The interrelationships of all major groups of Platyhelminthes: phylogenetic evidence from morphology and moleculesBiological Journal of the Linnean Society, 1999
- Conservation evaluation and phylogenetic diversityBiological Conservation, 1992